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Dear Sir/Madam, I was recently reading an article which uses one of your antibodies. I cannot seem to locate this product on your website and was wondering if you could point me in the right direction. The article states using: "a mouse-monoclonal antibody against mammalian Pgp (C219; Abcam, Cambridge, MA, USA).... This antibody targets two Pgp epitopes that are largely conserved in the SMDR2 sequence" Another issue you may be able to help me with is whether or not you have a primary antibody that would recognise a variety of pgp/mdr proteins (has to include MDR2 (human gene family ABCB1) and MRP1 (human gene family ABCC1)) i.e. it may bind a highly conserved region across the pgps. I would really appreciate any help with the matter as I have been trawling through many of the antibodies available and comparing immunogens. I hope you can help, thank you for your time Kind regards |
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ANSWER: |
Thank you for contacting us.
ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are
ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datasheets of these antibodies and select a product according to your requirements.
The anti MDR2 products are ab71792; ab24108 and ab111209
Anti MRP1 products are ab24102; ab63987; ab99531; ab81903
To search different products please type target name in the search box provided on the Abcam homepage at www.abcam.com. After hitting the search button the system will give you the sorted product as per your target request.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-ABCB4 antibody (ab71792) at 1/100 dilution + Mouse heart tissue lysates at 12.5 µg
Secondary
HRP-anti-rabbit
Predicted band size : 141 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40& 43 kDa,47 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab71792 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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