ABCB4 antibody (ab71792)

Product Name 

ABCB4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ABCB4

Immunogen 

A KLH conjugated synthetic peptide selected selected from the central region of human ABCB4.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects bands of approximately 40, 43, 47, 75 and 170 kDa (predicted molecular weight: 141 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human hepatocarcinoma tissue and mouse heart tissue lysates.

Cellular localization 

Integral membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export

Relevance 

ABCB4 is a membrane-associated protein and member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. The gene encoding this protein is a member of the MDR/TAP subfamily of genes. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. ABCB4 is a member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate.
ABCB4 is the energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells, though it is not capable of conferring drug resistance. This protein mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type III (PFIC3). PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for ABCB4 antibody images, references, products related to ab71792 and other tools.

ABCB4 antibody images:

References for ABCB4 antibody (ab71792)

  This product has been used in:

Gautier T et al. Hepatic lipid accumulation in apolipoprotein C-I-deficient mice is potentiated by cholesteryl ester transfer protein. J Lipid Res 48:30-40 (2007). WB, IHC-P, ELISA; Human, Mouse. PubMed: 17053273

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