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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - ABCB5 antibody (ab77549)
Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg
Predicted band size : 90 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
Western blot - Anti-ABCB5 antibody (ab77549)
Anti-ABCB5 antibody (ab77549) at 0.3 µg/ml + Human skin Lysate in RIPA buffer. at 35 µg
developed using the ECL technique
Predicted band size : 90 kDa
Observed band size : 90 kDa
Primary incubation was 1 hour.
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