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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Overview
Product name
Anti-ABCB5 antibody
See all ABCB5 products (3) ...
Description
Goat polyclonal to ABCB5
Tested applications
Flow Cyt, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Horse, Cow, Chimpanzee
Immunogen
Synthetic peptide: C-QTQHRNTSKKAQ, corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3).
C-QTQHRNTS KKAQ
Positive control
A431 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Drug resistance >> P glycoproteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
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Western blot - ABCB5 antibody (ab77549)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.
Tissue specificity
Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 2 ABC transporter domains.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ2M3G0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABCB 5 antibody
- ABCB5 antibody
- ABCB5 P-gp antibody
see all
Database links
- Entrez Gene: 743269 Chimpanzee
- Entrez Gene: 340273 Human
- Omim: 611785 Human
- SwissProt: Q2M3G0 Human
- Unigene: 404102 Human
- Unigene: 658821 Human
Anti-ABCB5 antibody images:
Western blot - ABCB5 antibody (ab77549)
Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
References for Anti-ABCB5 antibody (ab77549)
ab77549 has not yet been referenced specifically in any publications.
Publishing research using ab77549? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"