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Western blot - ABCB5 antibody (ab77549)
(enlarge)
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Product Name
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ABCB5 antibody
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See all ABCB5 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ABCB5
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Immunogen
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Synthetic peptide: C-QTQHRNTSKKAQ, corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3).
BLAST 'C-QTQHRNTSKKAQ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Cow, Hrs
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 cell lysate.
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Cellular localization
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Cell membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Drug resistance >> P glycoproteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
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Relevance
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ABCB5 is a plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. ABCB5 is also responsible for the resistance to doxorubicin of a subset of malignant melanomas. ABCB5 has more recently been identified as a marker for human malignant-melanoma-initiating cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCB5 antibody images, references, products related to ab77549 and other tools.
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ABCB5 antibody images:
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Search PubMed (MEDLINE) for references to ABCB5
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ABCB5 antibody - more information
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Customer reviews (feedback) regarding ABCB5 antibody |
Customer FAQs regarding ABCB5 antibody |
Protocols for ABCB5 antibody |
Price and availability of products related to ABCB5 antibody |
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ABCB5 antibody - related products:
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ABCB5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCB5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - ABCB5 antibody (ab77549)