|
|
|
|
Western blot - ABCB5 antibody - N-terminal (ab80108)
(enlarge)
|
|
|
|
|
Product Name
|
ABCB5 antibody
|
| |
See all ABCB5 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ABCB5
|
|
Immunogen
|
KLH conjugated synthetic peptide selected from the N terminal region of human ABCB5
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/1000.
WB: 1/50 - 1/100. Predicted molecular weight: 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
K562 cell line lysate
|
|
Cellular localization
|
Cell membrane; Multi-pass membrane protein.
|
|
Research areas
|
Cancer >> Drug resistance >> P glycoproteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
|
|
Relevance
|
ABCB5 is a plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. ABCB5 is also responsible for the resistance to doxorubicin of a subset of malignant melanomas. ABCB5 has more recently been identified as a marker for human malignant-melanoma-initiating cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Ammonium Sulphate Precipitation
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
ab80108 is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
|
|
Form
|
Liquid
|
|
Concentration
|
0.250 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ABCB5 antibody images, references, products related to ab80108 and other tools.
|
| |
ABCB5 antibody images:
|
 Western blot - ABCB5 antibody - N-terminal (ab80108)
ABCB5 antibody (ab80108) at 1/50 dilution + K562 cell line lysates at 35 µg
Predicted band size : 90 kDa
Observed band size : 90 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to ABCB5
|
| |
ABCB5 antibody - more information
|
Customer reviews (feedback) regarding ABCB5 antibody |
Customer FAQs regarding ABCB5 antibody |
Protocols for ABCB5 antibody |
Price and availability of products related to ABCB5 antibody |
| |
ABCB5 antibody - related products:
|
|
|
| |
ABCB5 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ABCB5 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
