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Western blot - ABCB7 antibody (ab66359)
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Immunocytochemistry/ Immunofluorescence - ABCB7 antibody (ab66359)
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Product Name
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ABCB7 antibody
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See all ABCB7 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCB7
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ABCB7. (Peptide available as ab66358.)
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Ms, Cow, Dog, Hrs, RMk
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
ICC/IF: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Lysates:
Rat Thymus Tissue
Human Spinal Cord
HeLa Whole Cell - Staurosporine Treated (24hr, 500nM)
Jurkat Whole Cell
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Cellular localization
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Mitochondrion inner membrane
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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ABCB7 is a mitochondrial protein involved in the transport of heme from the mitochondria to the cystol. Defects in ABCB7 are the cause of X-linked sideroblastic anemia with ataxia (ASAT). ASAT is a recessive disorder characterized by mitochondrial iron deposition and excess of protoporphyrin in erythroid cells. ASAT is also characterized by an infantile to early childhood onset of non-progressive cerebellar ataxia and mild anemia with hypochromia and microcytosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB7 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCB7 antibody images, references, products related to ab66359 and other tools.
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ABCB7 antibody images:
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 Western blot - ABCB7 antibody (ab66359)
All lanes : ABCB7 antibody (ab66359) at 1 µg/ml
Lane 1 : Thymus (Rat) Tissue Lysate
Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 83 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - ABCB7 antibody (ab66359)
ICC/IF image of ab66359 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66359, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
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Search PubMed (MEDLINE) for references to ABCB7
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ABCB7 antibody - more information
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Customer reviews (feedback) regarding ABCB7 antibody |
Customer FAQs regarding ABCB7 antibody |
Protocols for ABCB7 antibody |
Price and availability of products related to ABCB7 antibody |
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ABCB7 antibody - related products:
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ABCB7 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCB7 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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