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Western blot - ABCB8 antibody (ab83194)
(enlarge)
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Product Name
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ABCB8 antibody
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See all ABCB8 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCB8
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Immunogen
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Synthetic peptide corresponding to a region within the internal sequence amino acids 540-589 (EPVLFGTTIM ENIRFGKLEA SDEEVYTAAR EANAHEFITS FPEGYNTVVG) of Human ABCB8 (NP_009119)
BLAST 'EPVLFGTTIM ENIRFGKLEA SDEEVYTAAR EANAHEFITS FPEGYNTVVG ' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal heart lysate.
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Cellular localization
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Mitochondrion inner membrane; Multi-pass membrane protein.
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Research areas
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Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Relevance
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ABCB8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCB8 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may involve the compartmentalization and transport of heme, as well as peptides, from the mitochondria to the nucleus and cytosol. ABCB8 may also play a role in the transport of phospholipids into mitochondrial membranes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCB8 antibody images, references, products related to ab83194 and other tools.
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ABCB8 antibody images:
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 Western blot - ABCB8 antibody (ab83194)
ABCB8 antibody (ab83194) at 1 µg/ml + Human fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to ABCB8
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ABCB8 antibody - more information
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Customer reviews (feedback) regarding ABCB8 antibody |
Customer FAQs regarding ABCB8 antibody |
Protocols for ABCB8 antibody |
Price and availability of products related to ABCB8 antibody |
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ABCB8 antibody - related products:
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ABCB8 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCB8 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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