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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Overview
Product name
Anti-ABCB8 antibody
See all ABCB8 products (4) ...
Description
Rabbit polyclonal to ABCB8
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 540-589 (EPVLFGTTIM ENIRFGKLEA SDEEVYTAAR EANAHEFITS FPEGYNTVVG) of Human ABCB8 (NP_009119)
EPVLFGTTIM ENIRFGKLEA SDEEVYTAAR EANAHEFITS FPEGYNTVVG
Positive control
Fetal heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Western blot - ABCB8 antibody (ab83194)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ABCB8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCB8 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may involve the compartmentalization and transport of heme, as well as peptides, from the mitochondria to the nucleus and cytosol. ABCB8 may also play a role in the transport of phospholipids into mitochondrial membranes.
Cellular localization
Mitochondrion inner membrane; Multi-pass membrane protein.
Alternative names
- ATP binding cassette sub family B member 8 antibody
- ATP binding cassette sub family B member 8 mitochondrial antibody
- ATP binding cassette sub family B (MDR/TAP) member 8 antibody
see all
Database links
- Entrez Gene: 11194 Human
- Entrez Gene: 74610 Mouse
- Entrez Gene: 362302 Rat
- Omim: 605464 Human
- SwissProt: Q9NUT2 Human
- SwissProt: Q9CXJ4 Mouse
- SwissProt: Q5RKI8 Rat
Anti-ABCB8 antibody images:
Western blot - ABCB8 antibody (ab83194)
Anti-ABCB8 antibody (ab83194) at 1 µg/ml + Human fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-ABCB8 antibody (ab83194)
ab83194 has not yet been referenced specifically in any publications.
Publishing research using ab83194? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"