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Products:Signal Transduction >> Metabolism >> Drug metabolism
Overview
Product name
Anti-ABCB9 antibody
See all ABCB9 products (4) ...
Description
Rabbit polyclonal to ABCB9
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residues 475 and 525 of human ABCB9 (NP_062571.1)
Positive control
HeLa, 293T and mouse NIH3T3 whole cell lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 84 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications.
Cellular localization
Integral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum.
Alternative names
- ABC transporter 9 protein antibody
- ABCB 9 antibody
- ATP binding cassette sub family B (MDR/TAP) member 9 antibody
see all
Database links
- Entrez Gene: 23457 Human
- Entrez Gene: 56325 Mouse
- Omim: 605453 Human
- SwissProt: Q9NP78 Human
- SwissProt: Q9JJ59 Mouse
- Unigene: 511951 Human
Anti-ABCB9 antibody images:
Western blot - ABCB9 antibody (ab86222)
All lanes : Anti-ABCB9 antibody (ab86222) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 84 kDa
Observed band size : 84 kDa
Exposure time : 10 seconds
Immunoprecipitation - ABCB9 antibody (ab86222)
Detection of human ABCB9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-ABCB9 antibody (ab86222)
ab86222 has not yet been referenced specifically in any publications.
Publishing research using ab86222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"