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Anti-ABCC11 antibody (ab76727) at 1 µg/ml + Recombinant tagged human ABCC11 fragment at 0.1 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 154 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein. Predicted band size of immunogen is 37 kDa.
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