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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Overview
Product name
Anti-ABCC11 antibody
See all ABCC11 products (4) ...
Description
Mouse monoclonal to ABCC11
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment: FVPIAVKGLT NSKSAVMRFK KFFLQESPVF YVQTLQDPSK ALVFEEATLS WQQTCPGIVN GALELERNGH ASEGMTRPRD ALGPEEEGNS LGPELHKINL, corresponding to amino acids 433-533 of Human ABCC11 (NP_115972) with tag.
FVPIAVKGLT NSKSAVMRFK KFFLQESPVF YVQTLQDPSK ALVFEEATLS WQQTCPGIVN GALELERNGH ASEGMTRPRD ALGPEEEGNS LGPELHKINL
Positive control
Recombinant tagged human ABCC11 fragment.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Western blot - ABCC11 antibody (ab76727)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 154 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ABCC11 belongs to the ABC transporter family, conjugate transporter (TC 3.A.1.208) subfamily. It contains two ABC transmembrane type-1 domains and two ABC transporter domains. ABCC11 participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides and enhances the cellular extrusion of cAMP and cGMP. It also stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. It probably functions to secrete earwax.
Cellular localization
Membrane; Multi-pass membrane protein.
Alternative names
- ABCC 11 antibody
- ATP binding cassette protein C11 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 11 antibody
see all
Database links
- Entrez Gene: 473275 Chimpanzee
- Entrez Gene: 85320 Human
- Omim: 607040 Human
- SwissProt: Q96J66 Human
Anti-ABCC11 antibody images:
Western blot - ABCC11 antibody (ab76727)
Anti-ABCC11 antibody (ab76727) at 1 µg/ml + Recombinant tagged human ABCC11 fragment at 0.1 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 154 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein. Predicted band size of immunogen is 37 kDa.
References for Anti-ABCC11 antibody (ab76727)
ab76727 has not yet been referenced specifically in any publications.
Publishing research using ab76727? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"