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Western blot - ABCC11 antibody (ab82152)
Western blot - ABCC11 antibody (ab82152)
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Product Name 

ABCC11 antibody

 

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Product type 

Primary antibodies

Description 

Goat polyclonal to ABCC11

Immunogen 

Synthetic peptide: C-KVVEFDRPEVLRK from the internal region (near the C terminus) of the human ABCC11 protein sequence according to NP_115972.2; NP_660187.1.
BLAST 'KVVEFDRPEVLRK' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Specificity 

This antibody is expected to recognize both reported isoforms (NP_115972.2; NP_660187.1).

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 154 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast and human testis lysates.

Cellular localization 

Membrane; Multi-pass membrane protein.

Research areas 

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases

Relevance 

ABCC11 belongs to the ABC transporter family, conjugate transporter (TC 3.A.1.208) subfamily. It contains two ABC transmembrane type-1 domains and two ABC transporter domains.

ABCC11 participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides and enhances the cellular extrusion of cAMP and cGMP. It also stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. It probably functions to secrete earwax.

 

Alternative names ABCC11 antibody (ab82152)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

85320    (Human)

Omim

    

607040    (Human)

SwissProt

    

Q96J66    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCC11 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ABCC11 antibody images, references, products related to ab82152 and other tools.

 

ABCC11 antibody images:

Product image  Western blot - ABCC11 antibody (ab82152)

Western blot - ABCC11 antibody (ab82152)

ABCC11 antibody (ab82152) at 0.5 µg/ml + human breast lysate in RIPA buffer at 35 µg
developed using the ECL technique

Predicted band size : 154 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 hour

 
 

Search PubMed (MEDLINE) for references to ABCC11

 

ABCC11 antibody - more information

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Products for Western blot

Loading controls

Lysates

 

Positive Controls for ABCC11 antibody

ab30086

  

Breast (Human) Membrane Lysate - adult normal tissue

ab30249

  

Testis (Human) Membrane Lysate - adult normal tissue

 

Compatible Secondaries for ABCC11 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other ABCC11 Antibodies

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ABCC11 antibody - other tools:

Contact Abcam with a Technical Enquiry about ABCC11 antibody

 

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