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Western blot - ABCC11 antibody (ab82152)
(enlarge)
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Product Name
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ABCC11 antibody
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See all ABCC11 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ABCC11
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Immunogen
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Synthetic peptide: C-KVVEFDRPEVLRK from the internal region (near the C terminus) of the human ABCC11 protein sequence according to NP_115972.2; NP_660187.1.
BLAST 'KVVEFDRPEVLRK' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognize both reported isoforms (NP_115972.2; NP_660187.1).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 154 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast and human testis lysates.
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Cellular localization
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Membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Relevance
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ABCC11 belongs to the ABC transporter family, conjugate transporter (TC 3.A.1.208) subfamily. It contains two ABC transmembrane type-1 domains and two ABC transporter domains.
ABCC11 participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides and enhances the cellular extrusion of cAMP and cGMP. It also stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. It probably functions to secrete earwax.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCC11 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCC11 antibody images, references, products related to ab82152 and other tools.
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ABCC11 antibody images:
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 Western blot - ABCC11 antibody (ab82152)
ABCC11 antibody (ab82152) at 0.5 µg/ml + human breast lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 154 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 hour
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Search PubMed (MEDLINE) for references to ABCC11
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ABCC11 antibody - more information
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Customer reviews (feedback) regarding ABCC11 antibody |
Customer FAQs regarding ABCC11 antibody |
Protocols for ABCC11 antibody |
Price and availability of products related to ABCC11 antibody |
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ABCC11 antibody - related products:
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ABCC11 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCC11 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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