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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Overview
Product name
Anti-ABCD1 antibody
See all ABCD1 products (2) ...
Description
Goat polyclonal to ABCD1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Horse, Dog
Immunogen
Synthetic peptide: EDMQRKGYSEQD, corresponding to amino acids 564-575 of Human ABCD1 (NP_000024.2)
EDMQRKGYSE QD
Positive control
Human brain (cerebellum) lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
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Western blot - ABCD1 antibody (ab77364)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
Involvement in disease
Defects in ABCD1 are the cause of adrenoleukodystrophy X-linked (X-ALD) [MIM:300100]. X-ALD is a peroxisomal metabolic disorder characterized by progressive multifocal demyelination of the central nervous system and by peripheral adrenal insufficiency (Addison disease). It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. Different clinical manifestations exist like: cerebral childhood ALD (CALD), adult cerebral ALD (ACALD), adrenomyeloneuropathy (AMN) and 'Addison disease only' (ADO) phenotype.
Note=The promoter region of ABCD1 is deleted in the chromosome Xq28 deletion syndrome which involves ABCD1 and the neighboring gene BCAP31.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
Cellular localization
Peroxisome membrane.
Target information above from: UniProt accessionP33897
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adrenoleukodystrophy protein antibody
- Aldgh antibody
- ABC42 antibody
see all
Database links
- Entrez Gene: 215 Human
- Omim: 300371 Human
- SwissProt: P33897 Human
- Unigene: 159546 Human
Anti-ABCD1 antibody images:
Western blot - ABCD1 antibody (ab77364)
All lanes : Anti-ABCD1 antibody (ab77364) at 1 µg/ml
Lane 1 : Cell lysate prepared from HEK293 mock transfected cells.
Lane 2 : Cell lysate prepared from HEK293 cells overexpressing human ABCD1.
Predicted band size : 83 kDa
References for Anti-ABCD1 antibody (ab77364)
ab77364 has not yet been referenced specifically in any publications.
Publishing research using ab77364? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"