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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Overview
Product name
Anti-ABCD4 antibody
See all ABCD4 products (5) ...
Description
Goat polyclonal to ABCD4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Chimpanzee
Immunogen
Synthetic peptide: C-RDDIDNPDQRISQD, corresponding to internal sequence amino acids 136-149 of Human ABCD4 (NP_005041.1).
C-RDDIDNPD QRISQD
Positive control
Jurkat and MOLT4 cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab81905 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Primary antibody notes
Reported variants represent identical protein: NP_064719.1 NP_064731.1 NP_064720.1 NP_064730.1
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
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Western blot - ABCD4 antibody (ab81905)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
Cellular localization
Peroxisome membrane.
Target information above from: UniProt accessionO14678
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC 41 antibody
- ABC41 antibody
- ABCD 4 antibody
see all
Database links
- Entrez Gene: 453032 Chimpanzee
- Entrez Gene: 490781 Dog
- Entrez Gene: 5826 Human
- Entrez Gene: 19300 Mouse
- Entrez Gene: 299196 Rat
- GenBank: NM_005050 Human
- Omim: 603214 Human
- SwissProt: O14678 Human
see all
Anti-ABCD4 antibody images:
Western blot - ABCD4 antibody (ab81905)
Anti-ABCD4 antibody (ab81905) at 0.3 µg/ml + Jurkat Lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 69 kDa
Observed band size : 69 kDa
Primary incubation was 1 hour.
References for Anti-ABCD4 antibody (ab81905)
ab81905 has not yet been referenced specifically in any publications.
Publishing research using ab81905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"