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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-ABCE1 antibody
See all ABCE1 products (4) ...
Description
Rabbit polyclonal to ABCE1
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Dog, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human ABCE1.
(Peptide available as ab32269.)
Positive control
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Adapters >> Transmembrane
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). We also observe a doublet band at ~100 kDa. We are unsure as to the identity of this doublet.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains.
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
Target information above from: UniProt accessionP61221
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Database links
- Entrez Gene: 422462 Chicken
- Entrez Gene: 475454 Dog
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Entrez Gene: 406324 Zebrafish
- Omim: 601213 Human
- SwissProt: P61221 Human
see all
Anti-ABCE1 antibody images:
Western blot - ABCE1 antibody (ab32270)
All lanes : Anti-ABCE1 antibody (ab32270) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - ABCE1 antibody (ab32270)
ICC/IF image of ab32270 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32270, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - ABCE1 antibody (ab32270)
All lanes : Anti-ABCE1 antibody (ab32270) at 1/250 dilution
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 67,70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCE1 antibody (ab32270)
Image courtesy of Human Protein Atlas ab32270 staining ABCE1 in human duodenum, showing a distinct cytoplasmic staining pattern. Paraffin embedded human duodenum tissue was incubated with ab32270 (1/300 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab32270 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-ABCE1 antibody (ab32270)
This product has been referenced in:
- Furuta Met al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB; Human.Read more (PubMed: 19843643) »
See 1 publication for this product
Publishing research using ab32270? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"