Overview

  • Product nameAnti-ABCE1 antibody
    See all ABCE1 primary antibodies
  • Description
    Rabbit polyclonal to ABCE1
  • Tested applicationsIHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Dog, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human ABCE1.

    (Peptide available as ab32269.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab32270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). We also observe a doublet band at ~100 kDa. We are unsure as to the identity of this doublet.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAntagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCE family.
      Contains 2 4Fe-4S ferredoxin-type domains.
      Contains 2 ABC transporter domains.
    • Cellular localizationCytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2' 5' oligoadenylate binding protein antibody
      • 2''-5''-oligoadenylate-binding protein antibody
      • 2'5' oligoadenylate binding protein antibody
      • ABC 38 antibody
      • ABC38 antibody
      • ABCE 1 antibody
      • ABCE1 antibody
      • ABCE1_HUMAN antibody
      • ATP binding cassette sub family E (OABP) member 1 antibody
      • ATP binding cassette sub family E member 1 antibody
      • ATP-binding cassette sub-family E member 1 antibody
      • HuHP 68 antibody
      • HuHP68 antibody
      • OABP antibody
      • Ribonuclease 4 Inhibitor antibody
      • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L inhibitor antibody
      • RLI antibody
      • RNase L Inhibitor antibody
      • RNASEL1 antibody
      • RNASELI antibody
      • RNS 4I antibody
      • RNS4I antibody
      see all

    Anti-ABCE1 antibody images

    • All lanes : Anti-ABCE1 antibody (ab32270) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µg

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 67 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab32270 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32270, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • All lanes : Anti-ABCE1 antibody (ab32270) at 1/250 dilution

      Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
      Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 67 kDa
      Observed band size : 67,70 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
    • Image courtesy of Human Protein Atlas ab32270 staining ABCE1 in human duodenum, showing a distinct cytoplasmic staining pattern. Paraffin embedded human duodenum tissue was incubated with ab32270 (1/300 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab32270 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

    References for Anti-ABCE1 antibody (ab32270)

    This product has been referenced in:
    • Furuta M  et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . Read more (PubMed: 19843643) »

    See 1 Publication for this product

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