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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-ABCE1 antibody
See all ABCE1 products (4) ...
Description
Goat polyclonal to ABCE1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish, Cynomolgus Monkey, Orangutan
Immunogen
Synthetic peptide: KLNSIKDVEQKK from the C terminus of the protein human ABCE1 sequence according to NP_002931.1
KLNSIKDVEQ KK
Positive control
A431 cell lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Adapters >> Transmembrane
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Western blot - ABCE1 antibody (ab77367)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
Peptide ELISA: Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains.
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
Target information above from: UniProt accessionP61221
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Database links
- Entrez Gene: 422462 Chicken
- Entrez Gene: 514991 Cow
- Entrez Gene: 475454 Dog
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Entrez Gene: 406324 Zebrafish
- Omim: 601213 Human
see all
Anti-ABCE1 antibody images:
Western blot - ABCE1 antibody (ab77367)
Anti-ABCE1 antibody (ab77367) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
References for Anti-ABCE1 antibody (ab77367)
ab77367 has not yet been referenced specifically in any publications.
Publishing research using ab77367? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"