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Western blot - ABCF1 antibody [ABC5H06] (ab50976)
![Western blot - ABCF1 antibody [ABC5H06] (ab50976)](/ps/datasheet/images/ab50976.gif)
(enlarge)
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Product Name
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ABCF1 antibody [ABC5H06]
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See all ABCF1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ABC5H06] to ABCF1
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Immunogen
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Recombinant human ABCF1 fragment
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Immunology >> Immune System Diseases >> Autoimmune
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Relevance
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ABCF1 is a member of the GCN20 subfamily of the ATP-binding cassette (ABC) protein family and associates with eIF2 and ribosomes to facilitate mRNA translation [RGD]. This protein, however, lacks the transmembrane domains which are characteristic of most ABC transporters.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 23 (Human)
| Omim | | 603429 (Human)
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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ABC5H06
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, 200mM Sodium chloride, 20mM Tris HCl, pH 8.0 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Concentrated and dialyzed culture medium (protein free medium) of hybridoma
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCF1 antibody [ABC5H06] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCF1 antibody [ABC5H06] images, references, products related to ab50976 and other tools.
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ABCF1 antibody [ABC5H06] images:
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 Western blot - ABCF1 antibody [ABC5H06] (ab50976)
ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
developed using the ECL technique
Predicted band size : 96 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to ABCF1
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ABCF1 antibody [ABC5H06] - more information
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Customer reviews (feedback) regarding ABCF1 antibody [ABC5H06] |
Customer FAQs regarding ABCF1 antibody [ABC5H06] |
Protocols for ABCF1 antibody [ABC5H06] |
Price and availability of products related to ABCF1 antibody [ABC5H06] |
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ABCF1 antibody [ABC5H06] - related products:
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ABCF1 antibody [ABC5H06] - other tools:
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Contact Abcam with a Technical Enquiry about ABCF1 antibody [ABC5H06] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - ABCF1 antibody [ABC5H06] (ab50976)](/index.html?datasheet=50976#ab50976.gif)
