![Western blot - ABCF1 antibody [ABC5H06] (ab50976) image](#ab50976.gif)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-ABCF1 antibody [ABC5H06]
See all ABCF1 products (5) ...
Description
Mouse monoclonal [ABC5H06] to ABCF1
Tested applications
WB, IP, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human ABCF1 fragment
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, 200mM Sodium chloride, 20mM Tris HCl, pH 8.0
Purity
Tissue culture supernatant
Purification notes
Concentrated and dialyzed culture medium (protein free medium) of hybridoma
Clonality
Monoclonal
Clone number
ABC5H06
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Western blot - ABCF1 antibody [ABC5H06] (ab50976)
![Western blot - ABCF1 antibody [ABC5H06] (ab50976) image](/ps/datasheet/Images/50/ab50976/ab50976.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).
IP: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
Target
Function
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains.
Post-translational
modifications
Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
Target information above from: UniProt accessionQ8NE71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU041969 antibody
- D17Wsu166e antibody
- ABC27 antibody
see all
Database links
- Entrez Gene: 23 Human
- Omim: 603429 Human
- SwissProt: Q8NE71 Human
- Unigene: 655285 Human
Anti-ABCF1 antibody [ABC5H06] images:
Western blot - ABCF1 antibody [ABC5H06] (ab50976)
Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
developed using the ECL technique
Predicted band size : 96 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
References for Anti-ABCF1 antibody [ABC5H06] (ab50976)
This product has been referenced in:
- Pechenino ASet al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB; Rat.Read more (PubMed: 21750230) »
See 1 publication for this product
Publishing research using ab50976? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"