Anti-ABCF1 antibody - C-terminal (ab176190)

Overview

  • Product name
    Anti-ABCF1 antibody - C-terminal
    See all ABCF1 primary antibodies
  • Description
    Rabbit polyclonal to ABCF1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human ABCF1 aa 694-724 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NE71

  • Positive control
    • NCI-H460 and NIH-3T3 cell line lysates; Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 95 kDa.

Target

  • Function
    Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
  • Cellular localization
    Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Images

  • Anti-ABCF1 antibody - C-terminal (ab176190) at 1/1000 dilution + NIH-3T3 cell line lysates at 35 µg

    Predicted band size: 95 kDa

  • Anti-ABCF1 antibody - C-terminal (ab176190) at 1/1000 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size: 95 kDa

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human testis tissue labeling ABCF1 with ab176190 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab176190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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