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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab86223? Please let us know so that we can cite the reference in this datasheet
ab86223 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ABCF1 antibody (ab86223)
All lanes : Anti-ABCF1 antibody (ab86223) at 0.025 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 96 kDa
Observed band size : 117 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunoprecipitation - ABCF1 antibody (ab86223)
Detection of human ABCF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86223 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 1 second.
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