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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-ABCF1 antibody
See all ABCF1 products (5) ...
Description
Rabbit polyclonal to ABCF1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 150 and 200 of human ABCF1 (NP_001020262.1)
Positive control
HeLa and 293T whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 96 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains.
Post-translational
modifications
Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
Target information above from: UniProt accessionQ8NE71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU041969 antibody
- D17Wsu166e antibody
- ABC27 antibody
see all
Database links
- Entrez Gene: 23 Human
- Omim: 603429 Human
- SwissProt: Q8NE71 Human
- Unigene: 655285 Human
Anti-ABCF1 antibody images:
Western blot - ABCF1 antibody (ab86223)
All lanes : Anti-ABCF1 antibody (ab86223) at 0.025 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 96 kDa
Observed band size : 117 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunoprecipitation - ABCF1 antibody (ab86223)
Detection of human ABCF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86223 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 1 second.
References for Anti-ABCF1 antibody (ab86223)
ab86223 has not yet been referenced specifically in any publications.
Publishing research using ab86223? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"