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Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HeLa lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HepG2 lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-ABCF1 antibody (ab89109) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 96 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Immunofluorescence of purified ab89109 to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]
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