Overview

  • Product nameAnti-ABCF1 antibodySee all ABCF1 primary antibodies ...
  • Description
    Mouse polyclonal to ABCF1
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-845 of Human ABCF1 (AAH34488.1)

  • Positive control
    • WB: HeLa lysate, transfected 293T lysate, HepG2 lysate IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
  • Target information above from: UniProt accession Q8NE71 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Anti-ABCF1 antibody images

  • Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HeLa lysate at 50 µg

    Predicted band size : 96 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HepG2 lysate at 50 µg

    Predicted band size : 96 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ABCF1 antibody (ab89109) at 1 µg/ml

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 96 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified ab89109 to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]

References for Anti-ABCF1 antibody (ab89109)

ab89109 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"