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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-ABCF1 antibody
See all ABCF1 products (5) ...
Description
Mouse polyclonal to ABCF1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-845 of Human ABCF1 (AAH34488.1)
Positive control
WB: HeLa lysate, transfected 293T lysate, HepG2 lysate IF: HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 96 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains.
Post-translational
modifications
Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
Target information above from: UniProt accessionQ8NE71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU041969 antibody
- D17Wsu166e antibody
- ABC27 antibody
see all
Database links
- Entrez Gene: 23 Human
- Omim: 603429 Human
- SwissProt: Q8NE71 Human
- Unigene: 655285 Human
Anti-ABCF1 antibody images:
Western blot - ABCF1 antibody (ab89109)
Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HeLa lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Western blot - ABCF1 antibody (ab89109)
Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HepG2 lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Western blot - ABCF1 antibody (ab89109)
All lanes : Anti-ABCF1 antibody (ab89109) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 96 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ABCF1 antibody (ab89109)
Immunofluorescence of purified ab89109 to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]
References for Anti-ABCF1 antibody (ab89109)
ab89109 has not yet been referenced specifically in any publications.
Publishing research using ab89109? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"