Overview
- Product nameAnti-ABCF1 antibodySee all ABCF1 primary antibodies ...
- DescriptionMouse polyclonal to ABCF1
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
Full length protein, corresponding to amino acids 1-845 of Human ABCF1 (AAH34488.1)
- Positive controlWB: HeLa lysate, transfected 293T lysate, HepG2 lysate IF: HeLa cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab89109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa. |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
Target
- FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains. - Post-translational
modificationsIsoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
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Database links
- Entrez Gene: 23 Human
- Omim: 603429 Human
- SwissProt: Q8NE71 Human
- Unigene: 655285 Human
Target information above from: UniProt accession
Q8NE71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ABC27 antibodyABC50 antibodyABCF1 antibody
- ABCF1_HUMAN antibodyATP binding cassette 50 (TNF alpha stimulated) antibodyATP binding cassette 50 antibodyATP binding cassette sub family F member 1 antibodyATP binding cassette, sub family F (GCN20), member 1 antibodyATP-binding cassette 50 antibodyATP-binding cassette sub-family F member 1 antibodyAU041969 antibodyD17Wsu166e antibodyEST123147 antibodyGCN20 antibodyTNF alpha stimulated ABC protein antibodyTNF-alpha-stimulated ABC protein antibodyTNFalpha inducible ATP binding protein antibodywu:fb79c06 antibodywu:fc39a05 antibodywu:fj94a08 antibodyzgc:85667 antibody
see all
Anti-ABCF1 antibody images
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Western blot - ABCF1 antibody (ab89109)Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HeLa lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?) -
Western blot - ABCF1 antibody (ab89109)Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HepG2 lysate at 50 µg
Predicted band size : 96 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?) -
Western blot - ABCF1 antibody (ab89109)All lanes : Anti-ABCF1 antibody (ab89109) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 96 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - ABCF1 antibody (ab89109)Immunofluorescence of purified ab89109 to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]
References for Anti-ABCF1 antibody (ab89109)
ab89109 has not yet been referenced specifically in any publications.
