Anti-ABCF1 antibody (ab89109)

Overview

  • Product nameAnti-ABCF1 antibodySee all ABCF1 primary antibodies ...
  • Description
    Mouse polyclonal to ABCF1
  • Tested applicationsFlow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-845 of Human ABCF1 (AAH34488.1)

  • Positive control
    • WB: HeLa lysate, transfected 293T lysate, HepG2 lysate IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Anti-ABCF1 antibody images

  • Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HeLa lysate at 50 µg

    Predicted band size : 96 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Anti-ABCF1 antibody (ab89109) at 1 µg/ml + HepG2 lysate at 50 µg

    Predicted band size : 96 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ABCF1 antibody (ab89109) at 1 µg/ml

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 96 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified ab89109 to ABCF1 on HeLa cell. [antibody concentration 10 ug/ml]

References for Anti-ABCF1 antibody (ab89109)

ab89109 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - saponin
Sample Human Cell (liver)
Specification liver
Gating Strategy whole population
Preparation Cell harvesting/tissue preparation method: 0.25% Trypsin/EDTA or 5mM EDTA
Sample buffer: medium
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Verified customer

Submitted Jul 10 2014

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"