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Overview

  • Product nameABCF3 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 14-685 of Human ABCF3, with N terminal His tag, 672 amino acids, MWt 77.3kDa, Uniprot: Q9NUQ8
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:reconstitution with 41 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesEIDGQVFDYVTGVLHSGSADFESVDDLVEAVGELLQEVSG DSKDDAGIRAVCQRMYNTLRLAEPQSQ GNSQVLLDAPI QLSKITENYDCGTKLPGLLKREQSSTVNAKKLEKAEARLK AKQEKRSEKDTLKTSN PLVLEEASASQAGSRKESRLES SGKNKSYDVRIENFDVSFGDRVLLAGADVNLAWGRRYGLV GRNGL GKTTLLKMLATRSLRVPAHISLLHVEQEVAGDD TPALQSVLESDSVREDLLRRERELTAQIAAGRAE GSEA AELAEIYAKLEEIEADKAPARASVILAGLGFTPKMQQQPT REFSGGWRMRLALARALFARPDL LLLDEPTNMLDVRAI LWLENYLQTWPSTILVVSHDRNFLNAIATDIIHLHSQRLD GYRGDFETFIKS KQERLLNQQREYEAQQQYRQHIQVFI DRFRYNANRASQVQSKLKMLEKLPELKPVDKESEVVMKFP DG FEKFSPPILQLDEVDFYYDPKHVIFSRLSVSADLES RICVVGENGAGKSTMLKLLLGDLAPVRGIRHA HRNLKI GYFSQHHVEQLDLNVSAVELLARKFPGRPEEEYRHQLGRY GISGELAMRPLASLSGGQKSRV AFAQMTMPCPNFYILD EPTNHLDMETIEALGRALNNFRGGVILVSHDERFIRLVCR ELWVCEGGGV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab94013 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ATP binding cassette sub family F member 3ATP binding cassette, sub family F (GCN20) member 3EST201864FLJ11198
  • RelevanceABCF3 belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Contains 2 ABC transporter domains. There are 2 named isoforms produced by alternative splicing.

    • ABCF3 protein (Tagged-His Tag) images

    • SDS-PAGE - ABCF3 protein (Tagged-His Tag) (ab94013)
      SDS-PAGE - ABCF3 protein (Tagged-His Tag) (ab94013)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ABCF3 protein (Tagged-His Tag) (ab94013)

    ab94013 has not yet been referenced specifically in any publications.

    Publishing research using ab94013 ? Please let us know so that we can cite the reference in this datasheet

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