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Western blot - ABCG1 antibody (ab36969)
Western blot - ABCG1 antibody (ab36969)
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Product Name 

ABCG1 antibody

 

See all ABCG1 antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ABCG1

Immunogen 

A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400).

Reacts with 

Ms

Predicted to react 
(species key)

Hu
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/2,500. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse peritoneal macrophages.

Cellular localization 

Endoplasmic reticulum membrane and Golgi Apparatus membrane; Multi-pass membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism

Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

Relevance 

ABCG1, one of five members of the ABCG subfamily, belongs to the large family of ATP-binding cassette (ABC) transporters. The protein is expressed in the plasma membrane of adipocytes, macrophages and other cell types. In these cells, ABCG1 facilitates cholesterol efflux to apoAI and/or high-density lipoprotein (HDL). The function of ABCG1 is linked to both obesity and atherosclerosis. First, studies in Abcg1 knockout mice showed that the gene deletion resulted in a significantly reduced size of fat cells. Second, the activation of ABCG1 expression promoted atherosclerotic lesion development while inactivation of macrophage ABCG1 expression reduced it in proatherogenic mice. In addition, ABCG1 plays a key role in lipoprotein formation by glia.

 

Alternative names ABCG1 antibody (ab36969)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9619    (Human)
11307    (Mouse)
85264    (Rat)

GeneCard

    

GC21P042492    (Human)

Omim

    

603076    (Human)

SwissProt

    

P45844    (Human)
Q64343    (Mouse)
Q9EPG9    (Rat)

Unigene

    

124649    (Human)
8398    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCG1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ABCG1 antibody images, references, products related to ab36969 and other tools.

 

ABCG1 antibody images:

Product image  Western blot - ABCG1 antibody (ab36969)

Western blot - ABCG1 antibody (ab36969)

All lanes : ABCG1 antibody (ab36969)

Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)

developed using the ECL technique

Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).

For all lanes, mouse peritoneal macrophages (40 µg) was used as the positive control.

 

References for ABCG1 antibody (ab36969)

Specific publication references   This product has been used in: (two most recent references)

Mauerer R et al. High glucose, unsaturated and saturated fatty acids differentially regulate expression of ATP-binding cassette transporters ABCA1 and ABCG1 in human macrophages. Exp Mol Med 41:126-32 (2009). WB; Human. PubMed: 19287193

DiBlasio-Smith EA et al. Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cells. J Transl Med 6:59 (2008). PubMed: 18925943
If you publish research using ab36969 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to ABCG1

 

ABCG1 antibody - more information

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