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ab97573 |
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Read our guarantee »Products:Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Anti-ABCG1 antibody
See all ABCG1 products (3) ...
Rabbit polyclonal to ABCG1
IHC-P, WBmore details
Reacts with
Mouse, Human
Predicted to work with
Rat
Synthetic peptide, corresponding to internal sequence amino acids 300-400 of Human ABCG1 (Peptide available as ab97573.)
Mouse peritoneal macrophages.
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8
Concentration information loading...
Protein A purified
Polyclonal
IgG
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Our Abpromise guarantee covers the use of ab36969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
WB: 1/2,500Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).Can be blocked with ABCG1 peptide (ab97573).
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
Target information above from: UniProt accessionP45844
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ABCG1 antibody (ab36969)
All lanes : Anti-ABCG1 antibody (ab36969)
Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
developed using the ECL technique
Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
For all lanes, mouse peritoneal macrophages (40 µg) was used as the positive control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969)

ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
This product has been referenced in:
See all 4 publications for this product
Publishing research using ab36969? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-ABCG1 antibody (ab36969)
Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
developed using the ECL technique
Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
For all lanes, mouse peritoneal macrophages (40 µg) was used as the positive control.

ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
1
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