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Anti-ABCG1 antibody (ab36969)

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Overview

Product name

Anti-ABCG1 antibody
See all ABCG1 products (3) ...

Description

Rabbit polyclonal to ABCG1

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide, corresponding to internal sequence amino acids 300-400 of Human ABCG1 (Peptide available as ab97573.)

Positive control

Mouse peritoneal macrophages.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

  • Western blot - ABCG1 antibody (ab36969)Western blot - ABCG1 antibody (ab36969) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.

Tissue specificity

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.

Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.

Target information above from: UniProt accessionP45844 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ABC transporter 8 antibody
  • Abc8 antibody
  • ABCG1 antibody
  • ABCG1_HUMAN antibody
  • ATP-binding cassette sub family G member 1 antibody
  • ATP-binding cassette sub-family G member 1 antibody
  • ATP-binding cassette transporter 8 antibody
  • ATP-binding cassette transporter member 1 of subfamily G antibody
  • ATP-binding cassette, sub family G WHITE member 1 antibody
  • homolog of Drosophila white antibody
  • MGC34313 antibody
  • White protein homolog antibody
  • White protein homolog ATP binding cassette transporter 8 antibody
  • WHITE1 antibody
  • wht1 antibody
see all

Database links

see all

Anti-ABCG1 antibody images:

  Western blot - ABCG1 antibody (ab36969)

Western blot - ABCG1 antibody (ab36969)

All lanes : Anti-ABCG1 antibody (ab36969)

Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)

developed using the ECL technique

Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).

For all lanes, mouse peritoneal macrophages (40 µg) was used as the positive control.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969)

ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-ABCG1 antibody (ab36969)

This product has been referenced in:

  • Li Det al. Adenosine monophosphate activated protein kinase regulates ABCG1-mediated oxysterol efflux from endothelial cells and protects against hypercholesterolemia-induced endothelial dysfunction. Arterioscler Thromb Vasc Biol 30:1354-62 (2010). WB; Human.Read more (PubMed: 20395595) »
  • Wang Fet al. Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice. J Clin Invest 120:3979-95 (2010). WB; Mouse.Read more (PubMed: 20978351) »

See all 4 publications for this product

Publishing research using ab36969? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"