Anti-ABCG5 antibody (ab69713)

Overview

• Product nameAnti-ABCG5 antibodySee all ABCG5 primary antibodies ...
• Description
  Rabbit polyclonal to ABCG5
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ABCG5.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab86402.)

• Positive controlThis antibody gave a positive signal in the following Lysates: Human Liver Tissue, HepG2 Whole Cell, SW480 Whole Cell, Caco 2 Whole Cell and Ramos Whole Cell

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Atherosclerosis
  • Lipid transport

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

Applications

Target

• FunctionTransporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
• Tissue specificityStrongly expressed in the liver, lower levels in the small intestine and colon.
• Involvement in diseaseDefects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
• Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
  Contains 1 ABC transmembrane type-2 domain.
  Contains 1 ABC transporter domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q9H222 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64240 Human
  • Omim: 605459 Human
  • SwissProt: Q9H222 Human
  • Unigene: 132992 Human

• Alternative names
  ABCG5 antibodyABCG5_HUMAN antibodyATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody

  ATP binding cassette sub family G member 5 antibodyATP-binding cassette sub-family G member 5 antibodySterolin 1 antibodySterolin-1 antibodySTSL antibody

  see all

Anti-ABCG5 antibody images

• 

  Western blot - ABCG5 antibody (ab69713)
  All lanes : Anti-ABCG5 antibody (ab69713) at 1 µg/ml
  
  Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
  Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
  Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
  Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 73 kDa
  Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - ABCG5 antibody (ab69713)
  ICC/IF image of ab69713 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69713, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.