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Western blot - ABF1 antibody (ab21760)
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Product Name
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ABF1 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ABF1
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Immunogen
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Fusion protein: RGLDDESGPT HGNDSGNHRH NEEDDVHTQM TKNYSDVVND EDINVAIANA VANVDSQSNN KHDGKDDDAT NNNDGQDNNT NNDHNNNSNI NNNNVGSHGI corresponding to amino acids 201-300 of S. cerevisiae ABF1.
BLAST 'RGLDDESGPT HGNDSGNHRH NEEDDVHTQM TKNYSDVVND EDINVAIANA VANVDSQSNN KHDGKDDDAT NNNDGQDNNT NNDHNNNSNI NNNNVGSHGI ' with  or 
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Reacts with
(species key)
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Sc
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 82 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
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Relevance
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ABF1 is a DNA binding protein with possible chromatin-reorganizing activity. It is involved in transcriptional activation, gene silencing, and DNA replication and repair.
ABF1 is a trans acting factor in the regulation of transcription and in DNA replication. It is involved in the transcriptional activation of a subset of ribosomal proteins genes. It binds the ARS elements found in many promoters, binding to the sequence 5'-TCN(7)ACG-3'.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 853748 (Saccharomyces cerevisiae)
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| SwissProt | | P14164 (Saccharomyces cerevisiae)
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABF1 antibody images, references, products related to ab21760 and other tools.
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ABF1 antibody images:
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 Western blot - ABF1 antibody (ab21760)
All lanes : ABF1 antibody (ab21760) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to ABF1
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ABF1 antibody - more information
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Customer reviews (feedback) regarding ABF1 antibody |
Customer FAQs regarding ABF1 antibody |
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ABF1 antibody - related products:
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ABF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABF1 antibody |
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