Recombinant Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6175(2)] to ALKBH1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ALKBH1 antibody [EPR6175(2)]
See all ALKBH1 primary antibodies -
Description
Rabbit monoclonal [EPR6175(2)] to ALKBH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ALKBH1 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- A431, Jurkat, A549, K562, Raw 264.7, and NIH3T3 cell lysates, Human breast carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6175(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 8846 Human
- Entrez Gene: 211064 Mouse
- Omim: 605345 Human
- SwissProt: Q13686 Human
- SwissProt: P0CB42 Mouse
- Unigene: 94542 Human
- Unigene: 26840 Mouse
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Alternative names
- ABH antibody
- ABH1 antibody
- alkB antibody
see all
Images
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All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell) transfected with siRNA specifically targeting ALKBH1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 43 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 120 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDaLanes 1 - 4: Merged signal (red and green). Green - ab128895 observed at 43 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab128895 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab128895 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
ab128895, at a dilution of 1/50, staining ALKBH1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab128895 has been referenced in 2 publications.
- Kawarada L et al. Telencephalon-specific Alkbh1 conditional knockout mice display hippocampal atrophy and impaired learning. FEBS Lett 595:1671-1680 (2021). PubMed: 33930188
- Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167:816-828.e16 (2016). WB ; Human . PubMed: 27745969