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RabMAbs (5471-1)

Anti-ABH1 antibody [EPR6176] (ab126596)

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Overview

  • Product nameAnti-ABH1 antibody [EPR6176]See all ABH1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6176] to ABH1
  • Tested applicationsICC/IF, WB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive controlJurkat, K562 and A549 cell lysates. Human kidney tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab126596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/100 - 1/250.
WB WB: 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP IP: 1/10 - 1/100.
IHC-P IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for or Flow Cyt.

    • Target

    • FunctionDioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localizationMitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.

      • Target information above from: UniProt accession Q13686 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          ABH antibodyABH1 antibodyalkB antibody
          AlkB, alkylation repair homolog 1 (E. coli) antibodyALKB1_HUMAN antibodyALKBH antibodyALKBH1 antibodyAlkylated DNA repair protein alkB homolog 1 antibodyAlkylation repair homolog 1 antibodyAlkylation repair, alkB homolog antibodyAlpha-ketoglutarate-dependent dioxygenase ABH1 antibodyDNA lyase ABH1 antibodyDNA oxidative demethylase ALKBH1 antibodyhABH antibody
        see all

      Anti-ABH1 antibody [EPR6176] images

      • Western blot - Anti-ABH1 antibody [EPR6176] (ab126596)
        Western blot - Anti-ABH1 antibody [EPR6176] (ab126596)
        All lanes : Anti-ABH1 antibody [EPR6176] (ab126596) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : A549 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size : 43 kDa
        Observed band size : 43 kDa
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABH1 antibody [EPR6176] (ab126596)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABH1 antibody [EPR6176] (ab126596)
        ab126596, at 1/50 dilution, staining ABH1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
      • Other-Anti-ABH1 antibody [EPR6176](ab126596)
        Other-Anti-ABH1 antibody [EPR6176](ab126596)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References for Anti-ABH1 antibody [EPR6176] (ab126596)

      ab126596 has not yet been referenced specifically in any publications.

      Publishing research using ab126596 ? Please let us know so that we can cite the reference in this datasheet

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