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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Direct Chemical Reversal
Overview
Product name
Anti-ABH1 antibody
See all ABH1 products (5) ...
Description
Rabbit polyclonal to ABH1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human ABH1.
(Peptide available as ab19826.)
Positive control
HeLa cells overexpressesing flag tagged ABH1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Direct Chemical Reversal
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Western blot - ABH1 antibody (ab18525)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
Target
Function
Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Cellular localization
Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
Target information above from: UniProt accessionQ13686
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABH antibody
- alkB antibody
- ALKB1_HUMAN antibody
see all
Database links
- Entrez Gene: 8846 Human
- Omim: 605345 Human
- SwissProt: Q13686 Human
- Unigene: 94542 Human
Anti-ABH1 antibody images:
Western blot - ABH1 antibody (ab18525)
All lanes : Anti-ABH1 antibody (ab18525) at 1/1000 dilution
Lane 1 : HeLa cells overexpressesing flag tagged ABH1
Lane 2 : HeLa cells overexpressesing flag tagged ABH1 with Control siRNA knockdown
Lane 3 : HeLa cells overexpressesing flag tagged ABH1 with siRNA knockdown of ABH1
Lysates/proteins at 100 µg per lane.
Predicted band size : 43 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ABH1 antibody (ab18525)
ab18525 has not yet been referenced specifically in any publications.
Publishing research using ab18525? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"