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Western blot - ABH1 antibody (ab88775)
(enlarge)
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Western blot - ABH1 antibody (ab88775)
(enlarge)
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Product Name
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ABH1 antibody
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See all ABH1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ABH1
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Immunogen
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Full length Human ABH1 (AAH25787.1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate; ABH1 transfected 293T cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Direct Chemical Reversal
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Relevance
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ABH1 (also known as ALKB) is a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. Repair of DNA damage is essential for maintaining genome integrity, and repair deficiencies in mammals are associated with cancer, neurological disease and developmental defects. Alkylation damage in DNA is repaired by at least three different mechanisms, including damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine by E. coli AlkB.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABH1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABH1 antibody images, references, products related to ab88775 and other tools.
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ABH1 antibody images:
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 Western blot - ABH1 antibody (ab88775)
ABH1 antibody (ab88775) at 1/500 dilution + Jurkat cell lysate at 50 µg
Predicted band size : 43 kDa
Observed band size : 43 kDa
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Western blot - ABH1 antibody (ab88775)
All lanes : ABH1 antibody (ab88775) at 1/500 dilution
Lane 1 : ABH1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 43 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to ABH1
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ABH1 antibody - more information
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Customer reviews (feedback) regarding ABH1 antibody |
Customer FAQs regarding ABH1 antibody |
Protocols for ABH1 antibody |
Price and availability of products related to ABH1 antibody |
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ABH1 antibody - related products:
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ABH1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABH1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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