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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Direct Chemical Reversal
Overview
Product name
Anti-ABH1 antibody [hABH1-151]
See all ABH1 products (5) ...
Description
Mouse monoclonal [hABH1-151] to ABH1
Specificity
This antibody does not recognize hABH2 or hABH3.
Tested applications
Cross reactivity
Reacts with
Human, Monkey
Immunogen
Synthetic peptide: GE DAFRKLFRFY RQSR conjugated to KLH, corresponding to amino acids 19-34 of Human ABH1
GE DAFRKLFRFY RQSR
Positive control
A total cell extract of 293T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
hABH1-151
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Direct Chemical Reversal
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 43 kDa.
Target
Function
Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Cellular localization
Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
Target information above from: UniProt accessionQ13686
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABH antibody
- alkB antibody
- ALKB1_HUMAN antibody
see all
Database links
- Entrez Gene: 8846 Human
- Omim: 605345 Human
- SwissProt: Q13686 Human
- Unigene: 94542 Human
References for Anti-ABH1 antibody [hABH1-151] (ab49738)
ab49738 has not yet been referenced specifically in any publications.
Publishing research using ab49738? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"