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Overview

  • Product nameABH1 protein (His-DHFR tag)See all ABH1 proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 8-216 of Human ABH1 with N terminal His-DHFR tag; Predicted MWt 23.9 kDa excluding tag (BC025787).
  • Uniprot accessionQ13686
  • Molecular weight23.900kDa
  • Protein length209 amino acids
  • Expression hostE. coli

    • Properties

  • Purity 90 % Purified via His tag
  • Purification notesPurity determined to be 90% by SDS-PAGE.
  • E.C. Number1.14.11.-
  • FormLyophilised:Reconstitute with water to desired concentration.
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferConstituents: 0.32% Tris HCl, 0.58% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab127495 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        ABHABH1alkB
        AlkB, alkylation repair homolog 1 (E. coli)ALKB1_HUMANALKBHALKBH1Alkylated DNA repair protein alkB homolog 1Alkylation repair homolog 1Alkylation repair, alkB homologAlpha-ketoglutarate-dependent dioxygenase ABH1DNA lyase ABH1DNA oxidative demethylase ALKBH1hABH
      see all
  • FunctionDioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localizationMitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.

    • Target information above from: UniProt accession Q13686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for ABH1 protein (His-DHFR tag) (ab127495)

    ab127495 has not yet been referenced specifically in any publications.

    Publishing research using ab127495 ? Please let us know so that we can cite the reference in this datasheet

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