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Overview

  • Product nameABH2 proteinSee all ABH2 proteins and peptides ...
  • Protein descriptionRecombinant full length Human ABH2 (amino acids 1-261) with N terminal His tag; 281 amino acids with tag, Predicted MWt 31.4 kDa (UniProt Q6NS38).
  • Uniprot accessionQ6NS38
  • Molecular weight31.400kDa inclusive of tags
  • Protein length261 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab105622 was purified using conventional chromatography techniques.
  • Mass spectrometryMALDI-TOF
  • E.C. Number1.14.11.-
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 30% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
  • Concentration information loading...
  • SequenceMGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQ EPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHI RAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQ VFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLER IRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAH GSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTK K
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab105622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    MS MS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab154859.

    • Protein info

    • Alternative names
        2OG Fe(II) oxy DC19530023G02ABH 2
        ABH2alkB alkylation repair homolog 2ALKB2_HUMANALKBH2ALKBH2 alkBAlkylated DNA repair protein alkB homologAlkylated DNA repair protein alkB homolog 2Alkylation repair homolog 2Alpha ketoglutarate dependent dioxygenase alkB homolog 2Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2AU016977DNA oxidative demethylase ALKBH2EC 1.14.11.FLJ99103hABH2mABH2MGC90512Oxy DC1
      see all
  • FunctionDioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificityDetected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • Sequence similaritiesBelongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localizationNucleus. Detected in replication foci during S-phase.

    • Target information above from: UniProt accession Q6NS38 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ABH2 protein images

    • SDS-PAGE - ABH2 protein (ab105622)
      SDS-PAGE - ABH2 protein (ab105622)
      15% SDS-PAGE analysis of ab105622 (3µg)

    References for ABH2 protein (ab105622)

    ab105622 has not yet been referenced specifically in any publications.

    Publishing research using ab105622 ? Please let us know so that we can cite the reference in this datasheet

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    This ABH2 protein lost activity after being stored at -20C for around 8 months.

    Thank you for your call today and letting us know about the trouble with ab105622.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"