Overview

  • Product name
  • Description
    Goat polyclonal to ABHD4
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Dog, Pig
  • Immunogen

    Synthetic peptide:

    RPTNPSEIRAP

    with a Cysteine residue linker, corresponding to internal sequence amino acids 177-187 of Human ABHD4.

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.
  • Sequence similarities
    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH4 antibody
    • Abhd4 antibody
    • ABHD4_HUMAN antibody
    • Abhydrolase domain containing 4 antibody
    • Abhydrolase domain-containing protein 4 antibody
    • Alpha/beta hydrolase 4 antibody
    • Alpha/beta hydrolase domain-containing protein 4 antibody
    • Alpha/beta-hydrolase 4 antibody
    • FLJ12816 antibody
    • Lyso N acylphosphatidylethanolamine lipase antibody
    • Lyso-N-acylphosphatidylethanolamine lipase antibody
    • Protein ABHD4 antibody
    see all

Images

  • ab115473 at 3.75ug/ml staining ABHD4 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115473 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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