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Western blot - ABI2 antibody (ab70144)
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Immunocytochemistry/ Immunofluorescence - ABI2 antibody (ab70144)
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Product Name
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ABI2 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ABI2
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Immunogen
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ABI2 (NP_005750.4, 475 a.a), full-length human protein.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ABI2 transfected 293T cell lysate
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Cellular localization
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Isoform 1: Cell projection, lamellipodium and filopodium. Cytoplasm, cytoskeleton. Note: Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
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Relevance
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ABI2 (Abelson interactor 2) belongs to the ABI family. The Abelson interactors are among a group of proteins that interact with Abl tyrosine kinase, a non-receptor tyrosine kinase whose activation results in the regulation of cytoskeleton function, cell growth and apoptosis in response to a variety of biological stimuli.
ABI2 may act in regulation of cell growth and transformation by interacting tyrosine kinases ABL1 and/or ABL2 and may be involved in cytoskeletal reorganization and regulation of the ABL1/c-Abl-mediated phosphorylation of MENA. ABI2 is also known to be a tumor suppressor and a cell migration inhibitor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.49 - 0.49 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABI2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABI2 antibody images, references, products related to ab70144 and other tools.
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ABI2 antibody images:
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 Western blot - ABI2 antibody (ab70144)
All lanes : ABI2 antibody (ab70144) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - ABI2 antibody (ab70144)
ab70144 at 10µg/ml staining ABl2 in Hela cells by Immunocytochemistry/Immunofluorescence.
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Search PubMed (MEDLINE) for references to ABI2
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ABI2 antibody - more information
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Customer reviews (feedback) regarding ABI2 antibody |
Customer FAQs regarding ABI2 antibody |
Protocols for ABI2 antibody |
Price and availability of products related to ABI2 antibody |
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ABI2 antibody - related products:
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ABI2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABI2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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