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Products:Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Overview
Product name
Anti-ABI2 antibody
See all ABI2 products (5) ...
Description
Mouse polyclonal to ABI2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
ABI2 (NP_005750.4, 475 a.a), full-length human protein.
Positive control
ABI2 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
ICC/IF: Use a concentration of 10 µg/ml
Target
Relevance
ABI2 (Abelson interactor 2) belongs to the ABI family. The Abelson interactors are among a group of proteins that interact with Abl tyrosine kinase, a non-receptor tyrosine kinase whose activation results in the regulation of cytoskeleton function, cell growth and apoptosis in response to a variety of biological stimuli. ABI2 may act in regulation of cell growth and transformation by interacting tyrosine kinases ABL1 and/or ABL2 and may be involved in cytoskeletal reorganization and regulation of the ABL1/c-Abl-mediated phosphorylation of MENA. ABI2 is also known to be a tumor suppressor and a cell migration inhibitor.
Cellular localization
Isoform 1: Cell projection, lamellipodium and filopodium. Cytoplasm, cytoskeleton. Note: Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips
Alternative names
- Abelson interactor 2 antibody
- ABI 2 antibody
- ABI2B antibody
see all
Database links
- Entrez Gene: 10152 Human
- Omim: 606442 Human
- SwissProt: Q9NYB9 Human
Anti-ABI2 antibody images:
Western blot - ABI2 antibody (ab70144)
All lanes : Anti-ABI2 antibody (ab70144) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ABI2 antibody (ab70144)
ab70144 at 10µg/ml staining ABl2 in Hela cells by Immunocytochemistry/Immunofluorescence.
References for Anti-ABI2 antibody (ab70144)
This product has been referenced in:
- Maddala Ret al. Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival. Dev Biol : (2011). WB; Mouse.Read more (PubMed: 21945075) »
See 1 publication for this product
Publishing research using ab70144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"