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Western blot - ABI3BP antibody (ab82802)
(enlarge)
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Product Name
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ABI3BP antibody
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See all ABI3BP antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABI3BP
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Immunogen
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Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (LINPHHDWTL PSHCPNDRFY TIRYREKDKE KKWIFQICPA TETIVENLKP) of Human ABI3BP (NP_056244)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate
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Research areas
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Cancer >> Tumor biomarkers >> Other
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Relevance
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ABI3BP (ABI gene family member 3-binding protein) contains 2 fibronectin type-III domains and is thought to interact with ABI3. It is expressed in brain, heart, lung, liver, pancreas, kidney and placenta. ABI3BP expression is dramatically reduced in human lung cancer cell lines and primary lung tumor, while it is predominantly expressed in normal conditions, suggesting that it is involved in prevention of tumorigenesis.
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ABI3BP antibody (ab82802) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABI3BP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABI3BP antibody images, references, products related to ab82802 and other tools.
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ABI3BP antibody images:
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 Western blot - ABI3BP antibody (ab82802)
ABI3BP antibody (ab82802) at 1 µg/ml + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 119 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa,35 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
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Search PubMed (MEDLINE) for references to ABI3BP
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ABI3BP antibody - more information
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Customer reviews (feedback) regarding ABI3BP antibody |
Customer FAQs regarding ABI3BP antibody |
Protocols for ABI3BP antibody |
Price and availability of products related to ABI3BP antibody |
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ABI3BP antibody - related products:
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ABI3BP antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABI3BP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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