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Products:Cancer >> Tumor biomarkers >> Other
Overview
Product name
Anti-ABI3BP antibody
See all ABI3BP products (3) ...
Description
Rabbit polyclonal to ABI3BP
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (LINPHHDWTL PSHCPNDRFY TIRYREKDKE KKWIFQICPA TETIVENLKP) of Human ABI3BP (NP_056244)
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
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Western blot - ABI3BP antibody (ab82802)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ABI3BP (ABI gene family member 3-binding protein) contains 2 fibronectin type-III domains and is thought to interact with ABI3. It is expressed in brain, heart, lung, liver, pancreas, kidney and placenta. ABI3BP expression is dramatically reduced in human lung cancer cell lines and primary lung tumor, while it is predominantly expressed in normal conditions, suggesting that it is involved in prevention of tumorigenesis.
Cellular localization
Secreted
Alternative names
- ABI gene family member 3 (NESH) binding protein antibody
- ABI gene family member 3 binding protein antibody
- ABI3BP antibody
see all
Database links
- Entrez Gene: 25890 Human
- Entrez Gene: 320712 Mouse
- Omim: 606279 Human
- SwissProt: Q7Z7G0 Human
- SwissProt: B2RWZ0 Mouse
Anti-ABI3BP antibody images:
Western blot - ABI3BP antibody (ab82802)
Anti-ABI3BP antibody (ab82802) at 1 µg/ml + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 119 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa,35 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
References for Anti-ABI3BP antibody (ab82802)
ab82802 has not yet been referenced specifically in any publications.
Publishing research using ab82802? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"