Anti-ABL2 antibody [1H1B11] (ab54209)

Overview

• Product nameAnti-ABL2 antibody [1H1B11]See all ABL2 primary antibodies ...
• Description
  Mouse monoclonal [1H1B11] to ABL2
• Tested applicationsWB, ELISA, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Ni-NTA purified truncated recombinant ABL2 expressed in E. Coli strain BL21 (DE3).

• Positive controlIF: HeLa cells. WB: ABL2 recombinant protein.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number1H1B11
• IsotypeIgG1
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Other

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

Applications

Target

• FunctionNon-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
• Tissue specificityWidely expressed.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• DomainContains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
• Post-translational
  modificationsPhosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
  Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
• Cellular localizationCytoplasm > cytoskeleton.

Target information above from: UniProt accession P42684 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 27 Human
  • Omim: 164690 Human
  • SwissProt: P42684 Human
  • Unigene: 159472 Human

• Alternative names
  Abelson murine leukemia viral oncogene homolog 2 antibodyAbelson related gene protein antibodyAbelson tyrosine-protein kinase 2 antibody

  Abelson-related gene protein antibodyABL2 antibodyABL2_HUMAN antibodyABLL antibodyARG antibodyTyrosine kinase ARG antibodyTyrosine protein kinase ABL2 antibodyTyrosine-protein kinase ARG antibodyv abl Abelson murine leukemia viral oncogene homolog 2 antibody

  see all

Anti-ABL2 antibody [1H1B11] images

• 

  Western blot - ABL2 antibody [1H1B11] (ab54209)
  Anti-ABL2 antibody [1H1B11] (ab54209) at 1/200 dilution + truncated ABL2 recombinant protein
  
  Predicted band size : 128 kDa
  Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - ABL2 antibody [1H1B11] (ab54209)
  Immunofluorescence analysis of ethanol fixed HeLa cells, showing cytoplasmic staining, using ab54209 (1/100) with IgG-FITC.