Overview

  • Product name
    Anti-ABL2 antibody - N-terminal
    See all ABL2 primary antibodies
  • Description
    Rabbit polyclonal to ABL2 - N-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ABL2 (N terminal). NP_001129472.
    Database link: P42684

  • Positive control
    • HeLa and Jurkat cell lysate. Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab198718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 116 kDa.

Target

  • Function
    Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain
    Contains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
  • Post-translational
    modifications
    Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
    Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson murine leukemia viral oncogene homolog 2 antibody
    • Abelson related gene protein antibody
    • Abelson tyrosine-protein kinase 2 antibody
    • Abelson-related gene protein antibody
    • ABL2 antibody
    • ABL2_HUMAN antibody
    • ABLL antibody
    • ARG antibody
    • Tyrosine kinase ARG antibody
    • Tyrosine protein kinase ABL2 antibody
    • Tyrosine-protein kinase ARG antibody
    • v abl Abelson murine leukemia viral oncogene homolog 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling ABL2 with ab198718 at 1/25 dilution.

  • All lanes : Anti-ABL2 antibody - N-terminal (ab198718) at 1/400 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 40 µg per lane.


    Predicted band size : 116 kDa


    Exposure time : 2 minutes

    Gel: 8% SDS-PAGE

References

ab198718 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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