Overview

  • Product name
  • Description
    Rabbit polyclonal to ABL2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55 -430 of the Human ABL2 protein (P42684).

  • Positive control
    • IMR32 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab126256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 128 kDa.

Target

  • Function
    Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain
    Contains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
  • Post-translational
    modifications
    Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
    Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson murine leukemia viral oncogene homolog 2 antibody
    • Abelson related gene protein antibody
    • Abelson tyrosine-protein kinase 2 antibody
    • Abelson-related gene protein antibody
    • ABL2 antibody
    • ABL2_HUMAN antibody
    • ABLL antibody
    • ARG antibody
    • Tyrosine kinase ARG antibody
    • Tyrosine protein kinase ABL2 antibody
    • Tyrosine-protein kinase ARG antibody
    • v abl Abelson murine leukemia viral oncogene homolog 2 antibody
    see all

Anti-ABL2 antibody images

  • Anti-ABL2 antibody (ab126256) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 128 kDa

References for Anti-ABL2 antibody (ab126256)

ab126256 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Primary tubular kidney cell cultures)
Total protein in input
2000 µg
Immuno-precipitation step
Protein G
Specification
Primary tubular kidney cell cultures
Username

Barbara Torsello

Verified customer

Submitted Apr 19 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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