Overview
Product nameABL2 protein (Active)See all ABL2 proteins and peptides ...
Protein descriptionRecombinant Human ABL2 fusion protein, expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Expression hostBaculovirus infected sf9 cells
Properties
Purification notesPurity determined to be > 70% by densitometry.
FormLiquid
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: 150mM Imidazole
Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab51259
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
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Application notesFuncS: Kinase activity assay. Specific activity determined to be 1218nmol/min/mg.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Abelson murine leukemia viral oncogene homolog 2Abelson related gene proteinAbelson tyrosine-protein kinase 2
Abelson-related gene proteinABL2ABL2_HUMANABLLARGTyrosine kinase ARGTyrosine protein kinase ABL2Tyrosine-protein kinase ARGv abl Abelson murine leukemia viral oncogene homolog 2
see all
FunctionNon-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
DomainContains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
Post-translational
modificationsPhosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
Cellular localizationCytoplasm > cytoskeleton.
ABL2 protein (Active) images
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SDS-PAGE - ABL2 protein (Active) (ab51259)
Sample Purity Data.
References for ABL2 protein (Active) (ab51259)
ab51259
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
