Anti-ABPHYL1 antibody (ab22022)

Overview

• Product nameAnti-ABPHYL1 antibody
• Description
  Mouse polyclonal to ABPHYL1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Corn
  
• Immunogen

  Fusion protein: FLLKPVRPSDVSRLCSRVLR, corresponding to amino acids 116/135 of Zea mays ABPHYL1

  Run BLAST with Run BLAST with
• General notesProduced from outbred CD1 mice

  
  

  This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 50% Glycerol
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Other Antibodies
  • Plant related targets

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• RelevanceABPHYL1 is a response regulator in Zea mays. Mutations of the ABPHYL1 gene affect the geometric pattern of leaf initiation, leading to plants with opposite leaf arrangements instead of the usual alternating pattern found in maize, a pattern termed decussate phyllotaxy. This pattern starts in the embryo, where the shoot apical meristem is enlarged.
• Database links
  • SwissProt: Q9FXQ7 Corn

• Alternative names
  Response regulator antibodyZmRR3 antibody

Anti-ABPHYL1 antibody images

• 

  Western blot - ABPHYL1 antibody (ab22022)
  All lanes : Anti-ABPHYL1 antibody (ab22022) at 1/1000 dilution
  
  Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
  Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
  
  Secondary
  Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
  
  Predicted band size : 15 kDa