![Immunocytochemistry - ACAA1 antibody [2H6BE7 ] (ab110289) image](#ACAA1-Primary-antibodies-ab110289-4.jpg)
![Flow Cytometry - ACAA1 antibody [2H6BE7 ] (ab110289) image](#ACAA1-Primary-antibodies-ab110289-5.jpg)
![Western blot - Anti-ACAA1 antibody [2H6BE7 ] (ab110289) image](#ACAA1-Primary-antibodies-ab110289-7.jpg)
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Product name
Anti-ACAA1 antibody [2H6BE7 ]
See all ACAA1 products (5) ...
Description
Mouse monoclonal [2H6BE7 ] to ACAA1
Tested applications
ICC, Flow Cyt, IP, WB, In-Cell ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Human liver mitochondria
Positive control
HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
2H6BE7
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
In-Cell ELISA: Use a concentration of 1 µg/ml.
Target
Pathway
Lipid metabolism; fatty acid metabolism.
Sequence similarities
Belongs to the thiolase family.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP09110
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- peroxisomal antibody
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
see all
Database links
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Entrez Gene: 24157 Rat
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- SwissProt: P07871 Rat
- SwissProt: P21775 Rat
see all
Anti-ACAA1 antibody [2H6BE7 ] images:
Immunocytochemistry - ACAA1 antibody [2H6BE7 ] (ab110289)
![Immunocytochemistry - ACAA1 antibody [2H6BE7 ] (ab110289)](/ps/datasheet/images/110/ab110289/ACAA1-Primary-antibodies-ab110289-4.jpg)
ab110289, at 5 µg/mL, staining ACAA1 in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).
Flow Cytometry - ACAA1 antibody [2H6BE7 ] (ab110289)
![Flow Cytometry - ACAA1 antibody [2H6BE7 ] (ab110289)](/ps/datasheet/images/110/ab110289/ACAA1-Primary-antibodies-ab110289-5.jpg)
ab110289, at 1 µg/mL, staining ACAA1 in HL-60 cells (blue) or using an isotype control (red).
Western blot - Anti-ACAA1 antibody [2H6BE7 ] (ab110289)
![Western blot - Anti-ACAA1 antibody [2H6BE7 ] (ab110289)](/ps/datasheet/images/110/ab110289/ACAA1-Primary-antibodies-ab110289-7.jpg)
All lanes : Anti-ACAA1 antibody [2H6BE7 ] (ab110289) at 1 µg/ml
Lane 1 : Human liver
Lane 2 : HepG2
Lane 3 : Rat liver
Lane 4 : Mouse liver
Lysates/proteins at 10 µg per lane.
Predicted band size : 44 kDa
References for Anti-ACAA1 antibody [2H6BE7 ] (ab110289)
ab110289 has not yet been referenced specifically in any publications.
Publishing research using ab110289? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"