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Anti-ACAA1 antibody [3F11] (ab87988)

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Overview

Product name

Anti-ACAA1 antibody [3F11]
See all ACAA1 products (5) ...

Description

Mouse monoclonal [3F11] to ACAA1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human, Recombinant Fragment

Predicted to work with

Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Monkey

Immunogen

Recombinant fragment corresponding to amino acids 217-316 (GCFQAEIVPV TTTVHDDKGT KRSITVTQDE GIRPSTTMEG LAKLKPAFKK DGSTTAGNSS QVSDGAAAIL LARRSKAEEL GLPILGVLRS YAVVGVPPD) of Human ACAA1 (NP_001598) linked to a proprietary tag.

GCFQAEIVPV TTTVHDDKGT KRSITVTQDE GIRPSTTMEG LAKLKPAFKK DGSTTAGNSS QVSDGAAAIL LARRSKAEEL GLPILGVLRS YAVVGVPPD

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human liver tissue lysate and HepG2 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

3F11

Isotype

IgG2a

Light chain type

kappa

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome

  • Western blot - ACAA1 antibody [3F11] (ab87988)Western blot - ACAA1 antibody [3F11] (ab87988) image (enlarge)

  • Western blot - ACAA1 antibody [3F11] (ab87988)Western blot - ACAA1 antibody [3F11] (ab87988) image (enlarge)

  • Western blot - ACAA1 antibody [3F11] (ab87988)Western blot - ACAA1 antibody [3F11] (ab87988) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab87988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Pathway

Lipid metabolism; fatty acid metabolism.

Sequence similarities

Belongs to the thiolase family.

Cellular localization

Peroxisome.

Target information above from: UniProt accessionP09110 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • peroxisomal antibody
  • 3 ketoacyl CoA thiolase peroxisomal antibody
  • 3-ketoacyl-CoA thiolase antibody
  • ACAA antibody
  • ACAA1 antibody
  • Acetyl CoA acyltransferase 1 antibody
  • Acetyl Coenzyme A acyltransferase 1 antibody
  • Acetyl-CoA acyltransferase antibody
  • Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3 oxoacyl Coenzyme A antibody
  • Beta ketothiolase antibody
  • Beta-ketothiolase antibody
  • MS718 antibody
  • Peroxisomal 3 oxoacyl CoA thiolase antibody
  • Peroxisomal 3 oxoacyl Coenzyme A thiolase antibody
  • Peroxisomal 3-oxoacyl-CoA thiolase antibody
  • PTHIO antibody
  • THIK_HUMAN antibody
  • THIO antibody
see all

Database links

see all

Anti-ACAA1 antibody [3F11] images:

  Western blot - ACAA1 antibody [3F11] (ab87988)

Western blot - ACAA1 antibody [3F11] (ab87988)

Anti-ACAA1 antibody [3F11] (ab87988) at 1 µg/ml + immunogen at 0.1 µg
developed using the ECL technique

Predicted band size : 44 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


Immunogen alone should give a band of 37 kDa.

  Western blot - ACAA1 antibody [3F11] (ab87988)

Western blot - ACAA1 antibody [3F11] (ab87988)

Anti-ACAA1 antibody [3F11] (ab87988) at 1 µg/ml + human liver tissue lysate at 25 µg
developed using the ECL technique

Predicted band size : 44 kDa
Observed band size : 44 kDa

  Western blot - ACAA1 antibody [3F11] (ab87988)

Western blot - ACAA1 antibody [3F11] (ab87988)

Anti-ACAA1 antibody [3F11] (ab87988) at 1 µg/ml + HepG2 cell lysate at 25 µg
developed using the ECL technique

Predicted band size : 44 kDa
Observed band size : 44 kDa

References for Anti-ACAA1 antibody [3F11] (ab87988)

ab87988 has not yet been referenced specifically in any publications.

Publishing research using ab87988? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"