Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Overview
Product name
Anti-ACAA1 antibody
See all ACAA1 products (5) ...
Description
Rabbit polyclonal to ACAA1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD) of Human ACAA1 (NP_001598).
ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD
Positive control
Human fetal brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P: Use at an assay dependent dilution.
Target
Pathway
Lipid metabolism; fatty acid metabolism.
Sequence similarities
Belongs to the thiolase family.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP09110
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- peroxisomal antibody
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
see all
Database links
- Entrez Gene: 508324 Cow
- Entrez Gene: 477023 Dog
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Entrez Gene: 24157 Rat
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
see all
Anti-ACAA1 antibody images:
Western blot - ACAA1 antibody (ab84635)
Anti-ACAA1 antibody (ab84635) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44 kDa
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 antibody (ab84635)
ab84635 staining ACAA1 in liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 antibody (ab84635)
IHC image of ab84635 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84635, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ACAA1 antibody (ab84635)
ab84635 has not yet been referenced specifically in any publications.
Publishing research using ab84635? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"