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Overview

  • Product nameACAA1 protein (Tagged-His Tag)See all ACAA1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-383 (383 amino acids) of Human ACAA1 with an N terminal His tag. Predicted mwt: 41 kDa; UniProt ID = P09110.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 90 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMQRLQVVLGHLRGPADSGWMPQAAPCLSGAPQASAADVVV VHGRRTAICRAGRGGFKDTT PDELLSAVMTAVLKDVNL RPEQLGDICVGNVLQPGAGAIMARIAQFLSDIPETVPLST VN RQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLA DRGNPGNITSRLMEKEKARDCLIP MGITSENVAERFGI SREKQDTFALASQQKAARAQSKGCFQAEIVPVTTTVHDDK GTKRSI TVTQDEGIRPSTTMEGLAKLKPAFKKDGSTTA GNSSQVSDGAAAILLARRSKAEELGLPI LGVLRSYAVV GVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFAS QAAYCVEKLR LPPEKVNPLGGAVALGHPLGCTG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92056 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        3 ketoacyl CoA thiolase peroxisomal3-ketoacyl-CoA thiolaseACAA
        ACAA1Acetyl CoA acyltransferase 1Acetyl Coenzyme A acyltransferase 1Acetyl-CoA acyltransferaseAcetyl-Coenzyme A acyltransferase 1 (peroxisomal 3 oxoacyl Coenzyme ABeta ketothiolaseBeta-ketothiolaseperoxisomalPeroxisomal 3 oxoacyl CoA thiolasePeroxisomal 3 oxoacyl Coenzyme A thiolasePeroxisomal 3-oxoacyl-CoA thiolasePTHIOTHIK_HUMANTHIO
      see all
  • PathwayLipid metabolism; fatty acid metabolism.
  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationPeroxisome.

    • Target information above from: UniProt accession P09110 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ACAA1 protein (Tagged-His Tag) images

    • SDS-PAGE - ACAA1 protein (Tagged-His Tag) (ab92056)
      SDS-PAGE - ACAA1 protein (Tagged-His Tag) (ab92056)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ACAA1 protein (Tagged-His Tag) (ab92056)

    ab92056 has not yet been referenced specifically in any publications.

    Publishing research using ab92056 ? Please let us know so that we can cite the reference in this datasheet

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