Overview
- Product nameAnti-ACADL antibody [7F5DD6]See all ACADL primary antibodies ...
- DescriptionMouse monoclonal [7F5DD6] to ACADL
- Tested applicationsWB, IP more details
- Species reactivityReacts with: Mouse, Rat
Does not react with
Human - Immunogen
Rat liver mitochondria
- Positive controlRat Liver Homogenate, Rat Liver Mitochondria, Mouse Liver Homogenate and Mouse Liver Mitochondria
Properties
- FormLiquid
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HBS -
Concentration information loading... - PurityAmmonium Sulphate Precipitation
- Purification notesPurity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
- Clonality Monoclonal
- Clone number7F5DD6
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab128566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
| IP | IP: Use at an assay dependent concentration. |
Target
- PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
- Involvement in diseaseDefects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting.
- Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
- Cellular localizationMitochondrion matrix.
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Database links
- Entrez Gene: 11363 Mouse
- Entrez Gene: 25287 Rat
- SwissProt: P51174 Mouse
- SwissProt: P15650 Rat
- Unigene: 2445 Mouse
- Unigene: 174 Rat
Target information above from: UniProt accession
P28330
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACAD4 antibodyACADL antibodyACADL_HUMAN antibody
- Acyl Coenzyme A dehydrogenase long chain antibodyAcyl-CoA dehydrogenase long chain antibodyFLJ94052 antibodyLCAD antibodyLong chain acyl CoA dehydrogenase antibodyLong-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
see all
Anti-ACADL antibody [7F5DD6] images
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![Western blot - Anti-ACADL antibody [MS#2 7F5DD6] (ab128566)](http://a.abcam.com/ps/datasheet/images/128/ab128566/ACADL-Primary-antibodies-ab128566-4.jpg)
Western blot - Anti-ACADL antibody [MS#2 7F5DD6] (ab128566)All lanes : Anti-ACADL antibody [7F5DD6] (ab128566) at 1 µg/ml
Lane 1 : Ladder
Lane 2 : Rat Liver Homogenate at 15 µg
Lane 3 : Rat Liver Mitochondria at 15 µg
Lane 4 : Rat H4IIE cell lysate at 15 µg
Lane 5 : Mouse Liver Homogenate at 15 µg
Lane 6 : Mouse Liver Mitochondria at 15 µg
Lane 7 : Mouse MEF cell lysate at 15 µg
Predicted band size : 47 kDa -
![Immunoprecipitation - Anti-ACADL antibody [MS#2 7F5DD6] (ab128566)](http://a.abcam.com/ps/datasheet/images/128/ab128566/ACADL-Primary-antibodies-ab128566-2.jpg)
Immunoprecipitation - Anti-ACADL antibody [MS#2 7F5DD6] (ab128566)Immunoprecipitation using ab128566 Lane 1: Ladder Lane 2: Rat Liver Homogenate.
References for Anti-ACADL antibody [7F5DD6] (ab128566)
ab128566 has not yet been referenced specifically in any publications.
