Anti-ACADM antibody [3B7BH7] (ab110296)

  Immunocytochemistry - ACADM antibody [3B7BH7] (ab110296)

Immunocytochemistry image of ab110296 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110296 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody [3B7BH7] (ab110296)

ACADM immunohistochemistry in Human cerebellum visualized with ab110296 at 1/1000. MCAD immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of MCAD immunoreactivity in the Purkinje cell bodies.

  Western blot - ACADM antibody [3B7BH7] (ab110296)

All lanes : Anti-ACADM antibody [3B7BH7] (ab110296) at 0.125 µg/ml

Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : H9C2 (rat cells) lysates
Lane 4 : MEF (mouse cells) lysates

Lysates/proteins at 15 µg per lane.


Predicted band size : 47 kDa

  Flow Cytometry - ACADM antibody [3B7BH7] (ab110296)

HL-60 cells were stained with 1 µg/mL ab110296 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.