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Western blot - ACADM antibody (ab13677)
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Product Name
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ACADM antibody
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See all ACADM antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ACADM
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Immunogen
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Synthetic peptide: RLIVAREHIDKYKN, corresponding to C terminal amino acids 408-421 of Human ACADM.
BLAST 'C-RLIVAREHIDKYKN' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
Peptide ELISA: antibody detection limit dilution 1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Heart Lysate
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Cellular localization
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Mitochondrial
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACADM antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ACADM antibody images, references, products related to ab13677 and other tools.
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ACADM antibody images:
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 Western blot - ACADM antibody (ab13677)
ACADM antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
developed using the ECL technique
Predicted band size : 48 kDa
Primary incubated for 1 hour.
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References for ACADM antibody (ab13677)
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This product has been used in:
Powelka AM et al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB; Mouse. PubMed: 16374519
If you publish research using ab13677 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ACADM
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ACADM antibody - more information
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Customer reviews (feedback) regarding ACADM antibody |
Customer FAQs regarding ACADM antibody |
Protocols for ACADM antibody |
Price and availability of products related to ACADM antibody |
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ACADM antibody - related products:
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ACADM antibody - other tools:
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Contact Abcam with a Technical Enquiry about ACADM antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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