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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-ACADM antibody
See all ACADM products (10) ...
Description
Goat polyclonal to ACADM
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: RLIVAREHIDKYKN, corresponding to C terminal amino acids 408-421 of Human ACADM.
C-RLIVAREH IDKYKN
Positive control
Human Heart Lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.01 - 0.1 µg/ml.Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
IHC-P: Use a concentration of 3 - 5 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
This enzyme is specific for acyl chain lengths of 4 to 16.
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in disease
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP11310
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ACAD 1 antibody
- ACAD1 antibody
see all
Database links
- Entrez Gene: 34 Human
- Entrez Gene: 11364 Mouse
- Omim: 607008 Human
- SwissProt: P11310 Human
- SwissProt: P45952 Mouse
- Unigene: 445040 Human
- Unigene: 10530 Mouse
Anti-ACADM antibody images:
Western blot - ACADM antibody (ab13677)
Anti-ACADM antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
developed using the ECL technique
Predicted band size : 48 kDa
Primary incubated for 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody (ab13677)
ab13677 (3.8 µg/ml) staining ACADM in paraffin-embedded human Liver. Steamed antigen retieval with citrate buffer (pH 6) was carried out.
References for Anti-ACADM antibody (ab13677)
This product has been referenced in:
- Powelka AMet al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB; Mouse.Read more (PubMed: 16374519) »
See 1 publication for this product
Publishing research using ab13677? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"