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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-ACADM antibody
See all ACADM products (10) ...
Description
Mouse polyclonal to ACADM
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length Human ACADM (NP_000007.1)
Positive control
Human liver or HepG2 cell lysate. Human kidney tissue. HeLa cells. Transfected 293T cell line
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 46 kDa.
ICC/IF: Use a concentration of 10 µg/ml
IHC-P: Use a concentration of 3 µg/ml
Target
Function
This enzyme is specific for acyl chain lengths of 4 to 16.
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in disease
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP11310
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ACAD 1 antibody
- ACAD1 antibody
see all
Database links
- Entrez Gene: 34 Human
- Omim: 607008 Human
- SwissProt: P11310 Human
- Unigene: 445040 Human
Anti-ACADM antibody images:
Western blot - ACADM antibody (ab88507)
Anti-ACADM antibody (ab88507) at 1 µg/ml + Human liver lysate at 50 µg/ml
Predicted band size : 46 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Western blot - ACADM antibody (ab88507)
Anti-ACADM antibody (ab88507) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size : 46 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Western blot - ACADM antibody (ab88507)
All lanes : Anti-ACADM antibody (ab88507) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody (ab88507)
Purified ab88507 at 3ug/ml staining ACADM in formailn-fixed, paraffin-embedded Human kidney tissue.
Immunocytochemistry/ Immunofluorescence - ACADM antibody (ab88507)
Purified ab88507 at 10ug/ml staining ACADM in HeLa cells by immunofluorescence.
References for Anti-ACADM antibody (ab88507)
ab88507 has not yet been referenced specifically in any publications.
Publishing research using ab88507? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"