Overview
- Product nameAnti-ACADM antibodySee all ACADM primary antibodies ...
- DescriptionMouse polyclonal to ACADM
- Tested applicationsWB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length Human ACADM (NP_000007.1)
- Positive controlHuman liver or HepG2 cell lysate. Human kidney tissue. HeLa cells. Transfected 293T cell line
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab88507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
| IHC-P | IHC-P: Use a concentration of 3 µg/ml. |
Target
- FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
- PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
- Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
- Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
- Cellular localizationMitochondrion matrix.
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Database links
- Entrez Gene: 34 Human
- Omim: 607008 Human
- SwissProt: P11310 Human
- Unigene: 445040 Human
Target information above from: UniProt accession
P11310
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACAD 1 antibodyACAD1 antibodyAcadm antibody
- ACADM_HUMAN antibodyAcyl coenzyme A dehydrogenase antibodyAcyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibodyFLJ18227 antibodyFLJ93013 antibodyFLJ99884 antibodyMCAD antibodyMCADH antibodyMedium chain acyl CoA dehydrogenase antibodyMedium chain fatty acyl CoA dehydrogenase antibodyMedium chain specific acyl CoA dehydrogenase antibodyMedium chain specific acyl CoA dehydrogenase mitochondrial antibodyMedium-chain specific acyl-CoA dehydrogenase antibodymitochondrial antibody
see all
Anti-ACADM antibody images
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Western blot - ACADM antibody (ab88507)Anti-ACADM antibody (ab88507) at 1 µg/ml + Human liver lysate at 50 µg/ml
Predicted band size : 46 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?) -
Western blot - ACADM antibody (ab88507)Anti-ACADM antibody (ab88507) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size : 46 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?) -
Western blot - ACADM antibody (ab88507)All lanes : Anti-ACADM antibody (ab88507) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody (ab88507)Purified ab88507 at 3ug/ml staining ACADM in formailn-fixed, paraffin-embedded Human kidney tissue. -
Immunocytochemistry/ Immunofluorescence - ACADM antibody (ab88507)Purified ab88507 at 10ug/ml staining ACADM in HeLa cells by immunofluorescence.
References for Anti-ACADM antibody (ab88507)
ab88507 has not yet been referenced specifically in any publications.
